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・ Sort (typesetting)
・ Sort (Unix)
・ Sort code
・ Sort Merge Generator
・ Sort Of
・ Sort of Revolution
・ Sort Sol
・ Sort sol
・ Sort, Lleida
・ Sort-en-Chalosse
・ Sort-merge join
・ Sorta
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・ Sortable list of islands of Western Australia
・ Sortal
Sortase
・ Sortase A
・ Sortase B
・ Sortavala
・ Sorte Ellen og hendes søn
・ Sorte Hest
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・ Sorteberg relay
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・ Sorted (film)
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・ Sorted array
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・ Sorted for E's & Wizz


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Sortase : ウィキペディア英語版
Sortase

Sortase refers to a group of prokaryotic enzymes that modify surface proteins by recognizing and cleaving a carboxyl-terminal sorting signal. For most substrates of sortase enzymes, the recognition signal consists of the motif LPXTG (Leu-Pro-any-Thr-Gly), then a highly hydrophobic transmembrane sequence, followed by a cluster of basic residues such as arginine. Cleavage occurs between the Thr and Gly, with transient attachment through the Thr residue to the active site Cys residue, followed by transpeptidation that attaches the protein covalently to cell wall components. Sortases occur in almost all Gram-positive bacteria and the occasional Gram-negative (e.g. ''Shewanella putrefaciens'') or Archaea (e.g. ''Methanobacterium thermoautotrophicum''), where cell wall LPXTG-mediated decoration has not been reported. Although sortase A, the "housekeeping" sortase, typically acts on many protein targets, other forms of sortase recognize variant forms of the cleavage motif, or that catalyze the assembly of pilins into pili.
== Reaction ==
The ''Staphylococcus aureus'' sortase is a transpeptidase that attaches surface proteins to the cell wall; it cleaves between the Gly and Thr of the LPXTG motif and catalyses the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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