翻訳と辞書 Words near each other ・ S-phase-promoting factor ・ S-Phone ・ S-Plan ・ S-plane ・ S-PLUS ・ S-process ・ S-PULSE ・ S-RAMP ・ S-rank ・ S-ribosylhomocysteine lyase ・ S-SCH ・ S-segment ・ S-specific spore photoproduct lyase ・ S-succinylglutathione hydrolase ・ S-TADIL J ・ S-tag ・ S-TEC ・ S-TEC Corporation ・ S-TEC Sentry ・ S-Tone Inc. ・ S-train ・ S-Train (Korail) ・ S-Type ・ S-Type (Music) ・ S-type asteroid ・ S-type star ・ S-unit ・ S-VHS ・ S-Video ・ S-VOX Foundation Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク S-tag ： ウィキペディア英語版 S-tag S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. The amino acid sequence of the S-tag is: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser. It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to. Moreover, the peptide alone is thought not to fold into a distinct structure. On DNA-level the S-tag can be attached to the N- or C-terminus of any protein. After gene expression, such a tagged protein can be detected by commercially available antibodies. ==References== 1. R.T. Raines et al., The S-Tag Fusion System for Protein Purification. Methods Enzymol. 326, 362-367 (2000) 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「S-tag」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.