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BacMam
Baculovirus gene transfer into Mammalian cells, known from scientific research articles as BacMam, is the use of baculovirus to deliver genes to mammalian cells. Baculoviruses are insect cell viruses that can be modified to express proteins in mammalian cells. The unmodified baculovirus is able to enter mammalian cells, however its genes are not expressed unless a mammalian recognizable promoter is incorporated upstream of a gene of interest. Both unmodified baculovirus and baculovirus modified with a mammalian promoter (BacMam) are unable to replicate in humans and are thus non infectious.
Invented by Dr. Frederick M. Boyce,〔(US patent #5,871,986 )〕 BacMam is a baculovirus-mediated gene transfer technique that has gained widespread use because of advantages when compared to other transfection methods, (for reviews see, Kost, T.A. ''et al'',). In addition, BacMam has been found to have inherent flexibility over stable cell lines, which has contributed to its adoption as a standard gene transfer technique.
==General Properties==
The BacMam gene delivery technology is a transient expression system, which facilitates expression of toxic gene products. It has a broad range of transduction including many primary cell types and stem cells. The baculoviral genome has a large capacity for foreign gene insert with up to 38 kb have been tried successfully. Simultaneous delivery of multiple genes to the same cell is feasible. There is little to no microscopically observable cytopathic effects of BacMam particles on mammalian cells. The level of gene expression can be adjusted by viral dose or chemical additions using histone deacetylase inhibitors. Transduction of cells is performed by liquid only addition and therefore BacMam is amenable to automated methods. Finally viruses are stable when stored at 4 °C in the dark for long periods of time.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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