翻訳と辞書
Words near each other
・ Abtavil
・ ABTB1
・ Abteh
・ Abteiberg Museum
・ Abteilung
・ Abteilungsleiter (NSDAP)
・ Abtenau
・ Abtenauer
・ Abthain
・ Abthorpe
・ Abtin
・ Abtlöbnitz
・ Abtolemus
・ Abtorsh
・ Abtouyour
ABTS
・ Abts
・ Abtsbessingen
・ Abtsdorf
・ Abtsdorfer See
・ Abtsgmünd
・ Abtsregt
・ Abtsrodaer Kuppe
・ Abtsteinach
・ Abtswind
・ Abtu
・ Abtu, Afghanistan
・ Abtut, Fars
・ Abtweiler
・ Abtwil


Dictionary Lists
翻訳と辞書 辞書検索 [ 開発暫定版 ]
スポンサード リンク

ABTS : ウィキペディア英語版
ABTS

| Section2 =
| Section3 =
}}
In biochemistry, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS is chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect for binding of molecules to each other.
It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme (such as horseradish peroxidase) or alone with blue multicopper oxidase enzymes (such as laccase or bilirubin oxidase). Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly follow the reaction kinetics of any hydrogen peroxide-producing enzyme, or to simply quantify the amount of hydrogen peroxide in a sample.

The formal reduction potentials for ABTS are high enough for it to act as an electron donor for the reduction of oxo species such as molecular oxygen and hydrogen peroxide, particularly at the less-extreme pH values encountered in biological catalysis. Under these conditions, the sulfonate groups are fully deprotonated and the mediator exists as a dianion..
:ABTS–· + e → ABTS2– E°′ = 0.67 V vs SHE
:ABTS + e → ABTS–· E°′ = 1.08 V vs SHE
This compound is chosen because the enzyme facilitates the reaction with hydrogen peroxide, turning it into a green and soluble end-product. Its new absorbance maximum of 420 nm light (ε = 3.6 × 104 M–1 cm–1) can easily be followed with a spectrophotometer, a common laboratory instrument.
It is sometimes used as part of a glucose estimating reagent when finding glucose concentrations of solutions such as blood serum.
ABTS is also frequently used by the food industry and agricultural researchers to measure the antioxidant capacities of foods. In this assay, ABTS is converted to its radical cation by addition of sodium persulfate. This radical cation is blue in color and absorbs light at 734 nm. The ABTS radical cation is reactive towards most antioxidants including phenolics, thiols and Vitamin C. During this reaction, the blue ABTS radical cation is converted back to its colorless neutral form. The reaction may be monitored spectrophotometrically. This assay is often referred to as the Trolox equivalent antioxidant capacity (TEAC) assay. The reactivity of the various antioxidants tested are compared to that of Trolox, which is a water-soluble analog of vitamin E.
== Applications for Functional Food Analysis ==

Based on the special chemical properties of formed free radicals. ABTS assay has been wide used for the anti-oxidant capacity determination of food products. For example, polyphenol compounds, which widely exist in fruit, can quench free radicals inside human body, thus prevent oxidative damage by free radicals. The antioxidant potency of plant extract or food product has been measured by ABTS assay. One example with detailed method is the antioxidant activity analysis of Hibiscus products. 〔Zhen, J., Villani, T. S., Guo, Y., Qi, Y., Chin, K., Pan, M. H., ... & Wu, Q. (2016). Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves. Food chemistry, 190, 673-680.〕

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
ウィキペディアで「ABTS」の詳細全文を読む



スポンサード リンク
翻訳と辞書 : 翻訳のためのインターネットリソース

Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.