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・ 2-hydroxyhexa-2,4-dienoate hydratase
・ 2-Hydroxyisocaproic acid
・ 2-hydroxyisoflavanone dehydratase
・ 2-hydroxymethylglutarate dehydrogenase
・ 2-Hydroxymuconate semialdehyde
・ 2-hydroxymuconate tautomerase
・ 2-Hydroxymuconate-6-semialdehyde dehydrogenase
・ 2-hydroxymuconate-semialdehyde hydrolase
・ 2-hydroxyphytanoyl-CoA lyase
・ 2-hydroxypropyl-CoM lyase
・ 2-hydroxypyridine 5-monooxygenase
・ 2-hydroxyquinoline 5,6-dioxygenase
・ 2-hydroxyquinoline 8-monooxygenase
・ 2-Imidazoline
・ 2-iminoacetate synthase
2-Iminothiolane
・ 2-in-1 PC
・ 2-inch Medium Mortar
・ 2-inch mortar
・ 2-Iodobenzoic acid
・ 2-Iodoxybenzoic acid
・ 2-Isopropyl-5-methyl-1-(2,6-dihydroxy-4-nonylphenyl)cyclohex-1-ene
・ 2-isopropylmalate synthase
・ 2-Ketoarginine methyltransferase
・ 2-lane expressway
・ 2-MDP
・ 2-Me-DET
・ 2-Mercaptoethanol
・ 2-Mercaptoindole
・ 2-Mercaptopyridine


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2-Iminothiolane : ウィキペディア英語版
2-Iminothiolane

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2-Iminothiolane is a cyclic thioimidate compound also known as Traut's reagent. It is a thiolating reagent that reacts with primary amine groups, such as those of amino acids, to form sulfhydryl groups.
== Application ==

2-Iminothiolane reacts witih primary amines efficienty at pH 7 to 9, creating aminidine compounds with a sulfhydryl group. Thus it allows for crosslinking or labeling of molecules such as proteins through use of disulfide or thioether conjugation. It was first used to thiolate a subunit of ribosome in ''E. coli'' in 1973 by Robert Traut, its namesake, and his colleagues.〔R. R. Traut et al., ''Methyl 4-mercaptobutyrimidate as a cleavable cross-linking reagent and its application to the Escherichia coli 30S ribosome'', ''Biochemistry'' 12, 1973, pp. 3266–3273, PMID 4581787.〕
It also reacts with aliphatic and phenolic hydroxyl groups at high pH, albeit at a much slower rate.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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