TimeSTAMP protein labelling
| TimeSTAMP protein labelling ： ウィキペディア英語版|
TimeSTAMP (Time – Specific Tag for the Age Measurement of Proteins) is a technique invented at the Roger Tsien lab at the University of California, San Diego in 2008. It is a technique that can specifically label the newly synthesized copies of the genetically tagged protein.
==Principle of the TimeSTAMP technique==
The TimeSTAMP technique for labelling newly synthesized proteins of interest and is based on drug–dependent preservation of epitope tags.
A simple diagram showing the strategy for drug–dependent epitope tagging of newly synthesized proteins can be found here: ().
In this technique, a tag that is present on all the proteins that are synthesized after the one-time administration of a small molecule drug. To achieve this goal, specific protease activity is incorporated to confer self–removing behaviour onto an epitope tag. Then, a corresponding protease inhibitor is used in order to block the removal of the tag. As a result, only those proteins synthesized after the application of inhibitor would be tagged.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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